摘要
目的探讨VEGF165在人肝癌细胞株HepG2体外形成血管生成拟态(Vasculogenic mimicry,VM)中的作用。方法三维细胞培养HepG2,观察其形成血管样通道的能力;构建pcDNA3.0-VEGF165和pcDNA3.0-VEGF165b真核表达质粒,以Lipofectamine2000转染HepG2细胞,RT-PCR及Westernblot检测转染后各组细胞VEGF165和VEGF165b的表达;三维培养转染后HepG2细胞,观察各组细胞形成血管生成拟态的能力。结果 HepG2细胞在三维细胞培养系统中可形成血管样通道;VEGF165及VEGF165b真核表达质粒转染HepG2细胞后,RT-PCR及Western blot均示VEGF165和VEGF165b表达分别上调,而转染VEGF165b质粒组VEGF165表达下调;三维细胞培养转染后的HepG2细胞,各组形成血管生成拟态的能力无明显区别。结论 HepG2细胞可于三维培养系统中形成管道结构,但VEGF165对HepG2细胞体外形成血管生成拟态的能力无明显影响。
Objective To detect the role of VEGF165 gene in vasculogenic mimicry formation of HepG2.Methods Two and three-dimensional culture was wed to observed the vasculogenic mimicry formation of HepG2.Eukaryotic expression plasmids pcDNA3.0-VEGF165 and pcDNA3.0-VEGF165b were constructed and transfected into HepG2 cell by Lipofectamine2000,then cell were cultured in the three-Dimensional matrix,the expression of VEGF165 and VEGF165b were detected by RT-PCR and Western blot.Results HepG2 cells could form vasculogenic mimicry in three-Dimensional matrix.The expression of VEGF165 and VEGF165b of HepG2 cells were up-regulation after transfected with their corresponding plasmids,but the vasculogenic mimicry formation of transfected HepG2 cells has no change.Conclusion HepG2 cells could form vasculogenic mimicry in three-Dimensional matrix,but the overexpression of VEGF165 and VEGF165b gene can not significantly influence the vasculogenic mimicry formation.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2010年第11期1245-1248,共4页
Cancer Research on Prevention and Treatment
