肺鳞癌化疗敏感度相关基因的cDNA阵列研究

cDNA Array Study on Chemosensitivity Associated Genes of Lung Squamous Cell Carcinoma

目的筛选与肺癌化疗敏感度相关的基因,为化疗个体化方案的制定提供遗传学方面的依据。方法收集肺癌新鲜组织标本,部分用于提取组织RNA,部分用于肿瘤药物敏感实验(MTT法),病理学诊断为中分化鳞癌。根据药物敏感实验结果分为高敏感组和低敏感组,进行cDNA阵列实验比较筛选差异表达基因;Western blot检测部分差异表达基因的表达情况。结果 MTT结果显示,对顺铂(DDP)和多西紫杉醇(TAX)联合作用敏感的有4例(高敏感组),不敏感的有4例(低敏感组),cDNA阵列结果显示化疗高敏感组和低敏感组的差异表达基因中有57个基因,其中差异表达显著的基因15个;高敏感组中c-myc的表达高于低敏感组,GST-π在化疗低敏感组中的表达高于高敏感组,与基因芯片中cDNA表达变化一致。结论在肺鳞癌化疗敏感度不同的病例中存在差异表达基因,这些基因可能成为肺癌化疗敏感度预测的生物标志物。

Objective The lung cancer chemosensitivity associated genes are screened to provide genetic basis for individual therapeutic project.Methods Fresh lung cancer tissue were collected,total tissue RNA were extracted,and tumor drug sensitivity experiment（MTT method） were conducted.All of pathological diagnosis were moderately differentiated squamous cell carcinoma,all of tissues were classified into high-sensitivity group and low-sensitivity group.cDNA array technique was applied to screen differentially expressed genes.Part of differentially expressed genes were assayed by Western blot.Results Four high-sensitivity cases and 4 low-sensitivity cases under combination of DDP and TAX were selected according MTT,cDNA array result showed that 57 differentially expressed genes existed between high-sensitivity group and low-sensitivity group,including 15 differentially significantly expressed genes.c-myc expressed higher in high-sensitivity group than low-sensitivity group,GST pi expressed higher in low-sensitivity group than high-sensitivity group,which was accord to cDNA array.Conclusion There were differentially expressed genes in lung cancer with different chemosensitivity,these gene might become biomarkers for prediction of chemosensitivity.