c-ski在胃癌组织中的表达与临床因素相关性分析

Relationship between the expression of c-ski in gastric cancer and the clinicopathological parameters

目的探讨c-ski在胃癌组织中的表达与临床因素相关性分析。方法采用RT-PCR法和免疫组化法检测癌旁组织和胃癌组织中c-ski蛋白及mRNA表达情况。结果胃癌组织c-ski蛋白阳性率和c-skimRNA水平明显高于癌旁组织(P<0.01);c-ski蛋白阳性率肠型胃癌明显高于弥漫型胃癌,差异具有统计学意义(P<0.05)。c-ski蛋白阳性率与患者的性别、肿瘤的大小、浸润深度、是否有淋巴结及远处转移、1年的生存时间无关。结论 c-ski在胃癌组织中表达明显升高,且在弥漫型胃癌和肠型胃癌表达中存在差异。

Objective To investigate the relationship between the expression of c-ski in human gastric cancer and the clinicopathological parameters. Methods The expression of c-ski protein and mRNA were detected by immunohis-tochemistry and reverse transcriptase-polymerase chain reaction in adjacent normal tissues （ n = 28） and gastric cancer tissues （ n = 28） . Results The positive rate of c-ski protein and the level of c-ski mRNA in gastric cancer tissues were significantly higher than those in adjacent normal tissues （ P 0. 01） . The positive rate of c-ski protein in intestinal gastric cancer was higher than that in diffuse gastric cancer （ P 0. 05） ,but it was not related to sex,depth of tumor invasion,tumor size,lymph node,distant metastasis and 1-year survival time （ P 0. 05） . Conclusion The c-ski was overexpression in gastric cancer tissues and the positive rate of c-ski was obviously different between intestinal gastric cancer and diffuse gastric cancer.