MMP-10在人膀胱癌T24细胞系体外侵袭中的作用

The role of matrix metalloproteinase-10(MMP-10) on the invasion of human bladder cancer T24 cell line in vitro

目的:探讨基质金属蛋白酶MMP-10在人膀胱癌细胞体外侵袭中的作用。方法:设计并合成针对MMP-10的siRNA和阴性对照siRNA,以脂质体转染法将其分别导入具有体外高侵袭潜能的T24细胞系,RT-PCR和Western blot法检测MMP-10基因及蛋白表达水平。Transwell体外侵袭实验比较干扰MMP-10前后T24细胞体外侵袭潜能的变化。结果:MMP-10-siRNA可有效沉默MMP-10基因的表达,并降低T24细胞系的体外侵袭能力。结论:MMP-10可能在膀胱癌侵袭转移过程中发挥重要作用,抑制MMP-10可为预防及治疗转移性膀胱癌提供新的思路。

Objective：To investigate the role of matrix metalloproteinase-10（MMP-10） on the invasion of human bladder cancer in vitro.Methods：siRNA against MMP-10 gene and negative control siRNA were synthesized,and transfected into human bladder cancer T24 cell line,which has high invasive potential in vitro,.The expression of MMP-10 was detected by RT-PCR and Western blot,while the invasive potential in vitro was also compared by transwell assay.Results：siRNA against MMP-10 gene effectively inhibited the expression of MMP-10 as well as the in vitro invasive potential of bladder cancer T24 cell.Conclusion：MMP-10 may promote the invasion and metastasis in bladder cancer.Inhibition of MMP-10 may provide a new basis for the prevention and treatment of metastatic bladder cancer.