苦参碱对胃癌细胞培养基上清液诱导的人脐静脉内皮细胞增殖及迁移的抑制作用

Effects of Matrine on the proliferation and migration of the human endothelial cell cultured with supernatant derived from gastric cancer cells

目的:研究苦参碱(Matrine)对人胃癌细胞SGC-7901培养基上清液诱导的人脐静脉内皮细胞ECV-304增殖及侵袭能力的影响。方法:人胃癌细胞SGC-7901培养基上清液诱导人脐静脉内皮细胞ECV-304细胞增殖,不同浓度苦参碱干预后,MTT法检测内皮细胞增殖抑制率;采用Transwell共培养模型观察苦参碱对SGC-7901诱导血管内皮细胞ECV-304迁移的抑制作用。结果:15μg/ml、30μg/ml、60μg/ml、120μg/ml、240μg/ml苦参碱干预48小时,ECV-304增殖抑制率分别为(20.8±1.3)%、(25.1±2.2)%、(40.9±1.1)%,(62.9±2.2)%,(77.2±1.9)%;呈剂量依赖(P<0.05)。对照组和苦参碱浓度在2μg/ml、4μg/ml、8μg/ml时内皮细胞迁移细胞数分别为350.8±51.3、230.0±13.7、144.1±12.6、53.6±4.9,呈剂量效应关系。结论:苦参碱可抑制人胃癌细胞诱导的人脐静脉内皮细胞增殖和迁移能力。

Objective：To study the effects of Matrine on the proliferation and migration of human endothelial cell cultured with supernatant derived from gastric cancer cells.Methods：EVC-304 cells were cultured with supernatant MA together.MTT was used to measure inhibitory effects of MA on the growth of EVC-304 cells;Transwell assay was applied to detect the inhibitory effect of Matrine on invasiveness of EVC-304 cells.Results：After treated with Matrine（15μg/ml,30μg/ml,60μg/ml,120μg/ml,240μg/ml,48h）,MTT assay revealed the rates of inhibition were（20.8 ± 1.3） %,（25.1 ± 2.2） %,（40.9 ± 1.1） %,（62.9 ± 2.2） %,（77.2 ± 1.9） % respectively;After treated with Matrine（2μg/ml,4μg/ml,8μg/ml,48h）,flow cytometry revealed the numbers of migration were 350.8 ± 51.3,230.0 ± 13.7,144.1 ± 12.6,53.6 ± 4.9 respectively in a dose-response relationship（P 0.05）.Conclusion：Matrine can inhibit proliferation and migration of human endothelial cell cultured with supernatant derived from gastric cancer cells.