男性血液和精液中微量元素含量与精子活力的相关性研究

Correlation of the contents of trace elements in male body fluids with sperm quality

目的:探讨男性血液和精液中微量元素的分布及其与男性精液参数的相关性。方法:采用BH-5100五通道原子吸收仪、YY-1001血铅仪和伟力彩色精子自动分析仪,对113例男性血液和精液进行钙、镁、铜、锌、铁、铅的测定及精液常规分析,以了解微量元素和精液参数的相互关系。结果:①血液与精液中铅、铜、锌、铁含量显著相关,其中以铜的相关系数最大;钙、镁没有相关性。②血液和精液中铅、铜、锌、钙、镁、铁6种微量元素分布存在显著性差异。③精液中钙与精子活率、直线运动精子活率、曲线速度、平均移动角度、活跃精子密度、直线速度、平均路径速度、侧摆幅度等指标显著正相关;铅与精子活率、直线运动精子活率、活跃精子密度、活动精子密度、直线运动精子密度等指标呈显著负相关;镁元素与精子活率、直线运动精子活率、直线运动精子密度呈显著负相关;精液酸碱度与钙、镁、铁3种元素呈显著负相关。④血液中的锌与精子活率和直线运动精子活率呈显著负相关,镁与精子活率、直线运动精子活率、平均路径速度呈显著负相关,铅与侧摆幅度呈显著正相关。结论:不同体液中的微量元素分布和精子的运动功能存在不同的相关性。

Objective：To explore the contents of trace elements in the blood and seminal plasma of men and their correlation with semen parameters.Methods：We detected and analyzed the contents of calcium,magnesium,copper,zinc,iron and lead in the blood and semen of 113 men with the BH-5100 5-channel atomic absorption spectrometer,YY-1001 blood lead instrument and Weili color sperm automatic analyzer to investigate the correlation of the contents of trace elements with quality.Results：The contents of lead,copper,zinc and iron in the blood were significantly correlated with those in the seminal plasma,but not the contents of calcium and magnesium.The contents of lead,copper,zinc,calcium,magnesium and iron in the blood were significantly different from those in the semen.In the seminal plasma,the content of calcium showed a significant positive correlation with sperm motility,linear motile sperm motility（LMSM）,straight line velocity（VSL）,curvilinear velocity（VCL）,mean angle of deviation（MAD）,active sperm density（ASD）,average path velocity（VAP）and lateral head amplitude（ALH）,while that of lead was negatively correlated with sperm motility,LMSM,ASD,motile sperm density（MSD）and linear motile sperm density（LMSD）,and so was that of magnesium with sperm motility,LMSM and LMSD,and those of calcium,magnesium and iron with seminal pH.In the blood,the content of zinc was negatively correlated with sperm motility and LMSM,and so was that of magnesium with sperm motility,LMSM and VAP,while that of lead was positively correlated with ALH.Conclusion：The contents of trace elements in different body fluids are differently correlated with sperm quality.