促红细胞生成素防治大鼠急性肾小管坏死的实验研究

Experimental study of the prevention and treatment of acute renal tubular necrosis with erythropoietin in rats

目的 探讨促红细胞生成素对大鼠急性肾小管坏死的防治作用.方法 按10 mL·kg^-1肌肉注射甘油建立大鼠急性肾小管坏死模型.30只Wistar大鼠随机（随机数字法）分为正常组、模型组、治疗组.治疗组予促红细胞生成素（1000 IU·kg^-1）尾静脉注射,实验结束时检测各组大鼠血尿素氮（Bun）、血肌酐（Scr）、尿渗量、尿N-乙酰-β-D-氨基葡萄糖苷酶（NAG）、尿β2微球蛋白（β2-MG）,肾组织匀浆丙二醇（MDA）和超氧化物歧化酶（SOD）,并进行肾脏组织学观察.结果 与模型组比较,治疗组大鼠Bun、Scr、尿NAG、尿β2-MG、肾组织匀浆MDA明显下降,而尿渗量、肾组织匀浆SOD升高,肾脏病理改变好转.结论 促红细胞生成素可能通过抗氧自由基损伤,提高内源性抗氧化能力达到对急性肾小管坏死大鼠肾脏的部分保护作用.

Objective To study the preventive effects of erythropoietin （EPO） on acute tubular necrosis of kidney in rats. Method The rat models of acute renal tubular necrosis were established with injecting glycerol in dose of 10 mL/kg. Thirty Wistar rats were randomly （random number） divided into control group, model group and EPO treatment group. EPO was administered intravenously into rats of treatment group in a dose of 1000IU/kg. Levels of blood urea nitrogen （BUN） and serum creatinine （Scr）, urine osmolality, urine N-acetyl-D-glucosaminidase （NAG）, urine β2-microglobulin （β2-MG）, tissue MDA and SOD of rats in the three groups were assayed after the experiment. Renal histological examination was also performed. Results Compared with model group, the levels of BUN and Scr, urine osmolality, NAG,β2-MG and tissue MDA in EPO treament group were significantly lower, but urine osmolality and tissue SOD of rats remarkably increased in comparison with model group. EPO also lessened the histological changes in treatment group. Conclusions EPO has some protective effects on acute renal tubular necrosis in rats, which is probably through preventing oxygen free radical damage and elevating endogenous antioxidation potential.