婴儿巨细胞病毒感染与自然杀伤细胞C型凝集素受体的表达

The relationship between expressions of C-type lectin receptors on natural killer cells and infant human cytomegalovirusinfection

目的 探讨自然杀伤（NK）细胞C型凝集素受体表达与婴儿人巨细胞病毒（HCMV）感染的关系.方法 2006年1月至2008年6月HCMV感染患儿79例和母乳性黄疸对照患儿39例.根据外周血中性粒细胞pp65抗原血症结果,分为活动性HCMV感染组48例和非活动性HCMV感染组31例,并对活动性HCMV感染组的48例婴儿进行更昔洛韦治疗2周.应用流式细胞术检测外周血NK细胞C型凝集素受体NKG2A、NKG2C、NKG2D表达.三组数据比较采用Kruskal-Wallis独立样本非参数检验,两组之间比较采用Mann-Whiteney配对样本非参数检验.结果 NK细胞抑制性受体NKG2A表达在活动性HCMV感染组、非活动性HCMV感染组和对照组之间差异无统计学意义（Х^2=3.95,P＞0.05）;NK细胞活化性受体NKG2C、NKG2D表达在活动性HCMV感染组、非活动性HCMV感染组和对照组之间差异有统计学意义（Х^2=24.91,P＜0.01;Х^2=47.80,P＜0.01）.NK细胞活化性受体NKG2C、NKG2D表达在HCMV感染组较对照组明显升高（Z=-4.72,P＜0.01;Z=-5.15,P＜0.01）.NK细胞活化性受体NKG2D表达在活动性HCMV感染组较非活动性HCMV感染组明显升高（Z=-5.08,P＜0.01）.更昔洛韦治疗后NK细胞活化性受体NKG2D表达降低（Z=-1.34,P=0.07）.结论 在调节NK细胞功能和婴儿抗HCMV免疫方面,NKG2C和NKG2D表达可能起重要作用.

Objective To explore the relationship between expressions of C-type lectin receptors on natural killer（NK） cells and infant human cytomegalovirus （HCMV） infection. Methods Seventynine cases of HCMV infection infants and 39 cases of HCMV non-infection control infants admitted during January 2006 to June 2008 were recruited in this study. According to HCMV pp65 antigenemia levels in the peripheral blood, 79 cases of HCMV infection infants were divided into two groups： 48cases of active HCMV infection and 31 cases of inactive HCMV infection. The 48 cases of infants with active HCMV infection were treated with ganciclovir for 2 weeks. The expressions of NKG2A,NKG2C, and NKG2D receptors on NK cells in the peripheral blood were examined by flow cytometry.Data analysis was done using SPSS 17.0 software. Comparisons among 3 groups were performed by Kruskal-Wallis nonparametric test for independent samples and comparisons between groups were done by Mann-Whiteney nonparametric test for paired samples. Results There was no difference of the inhibitory receptor NKG2A expression on NK cells among groups of active HCMV infection, inactive HCMV infection and HCMV non-infection controls （Х^2 = 3. 95, P〉0. 05）. However, there was obvious difference of activating receptors of NKG2C and NKG2D expressions on NK cells among the three groups （Х^2 =24.91 and Х^2 =47. 80, respectively; both P〈0.01）. The expressions of NKG2C and NKG2D on NK cells in the HCMV infection group were both higher than the control group （Z=-4.72 and Z=-5.15, respectively; both P〈0.01）. The expression of NKG2D on NK cells in the active HCMV infection group was higher than that in the inactive HCMV infection group （Z= -5.08,P〈0.01）. The expression of NKG2D on NK cells decreased after ganciclovir treatment （Z= - 1.34,P=0. 07）. Conclusion Expressions of NKG2C and NKG2D on NK cells might play a significant role in regulating NK cell function and anti-HCMV immunity in infants.