辅助性T淋巴细胞17在肝囊型包虫病患者外周血中的变化

The change of T helper 17 cells in the peripheral blood monouclear cells in patients with liver cystic echinococcosis

目的 探讨肝囊型包虫病患者外周血中辅助性T淋巴细胞（Th）17的变化.方法 将56例受试者分为：健康志愿者（HD）组20例,肝囊型包虫病（CE）组18例,肝囊型包虫病合并胆瘘（BF）组18例.流式细胞仪胞内染色分析法检测各组受试者外周血中Th17细胞占CD4^＋T淋巴细胞比例,ELlSA检测血清Th17细胞相关细胞因子IL-17、IL-23水平.数据行t检验和Pearson相关分析.结果 CE组患者外周血Th17/CD4^＋T淋巴细胞比例为（0.23±0.11）%,明显低于BF组的（0.76±0.43）%和HD组的（0.52±0.50）%（t=2.225,t=4.077,均P＜0.05）,而BF组和HD组间差异无统计学意义（t=1.931,P＞0.05）.血清IL-17和IL-23在CE组分别为（12.1±3.7）ng/L和（84.4±46.0）ng/L,明显低于BF组的（15.5±4.1）ng/L和（138.6±37.9）ng/L（t=2.515,t=3.649,均P＜0.05）,也低于HD组的（14.8±4.4）ng/L和（138.1±48.7）ng/L（t=2.401,t=3.706,均P＜0.05）,而BF组和HD组间差异无统计学意义（t=0.534,P＞0.05）.各组血清IL-17水平与IL-23水平呈正相关（r=0.657,P＜0.05）.结论 肝囊型包虫病患者外周血中Th17细胞比例明显减少,血清IL-17、IL-23水平显著降低.

Objective To investigate the change of T helper 17 cells （Th17） in the peripheral blood mononuclear cell （PBMC） in patients with liver cystic echinococcosis. Methods Fifty-six subjects were divided into three groups： healthy controls （HD, n = 20）, patients with cystic echinococcosis （CE, n= 18） and patients with cystic echinococcosis combined with bile fistula （BF,n= 18）. The frequency of Th17 cells in CD4＋ T lymphocytes was detected by flow cytometry. Th17-related cytokines including interleukin （IL）-17 and IL-23 were measured by enzyme-linked immunosorbent assay （ELISA）. The data were analyzed by t test and Pearson correlation analysis.Results The frequency of Th17 in the peripheral blood was significantly lower in CE group compared to BF group and HD group [（0. 23±0. 11）% vs （0. 76±0.43）% vs （0.52±0.50）%; t=2. 225 and4. 077 respectively, both P〈0.05）, while there was no statistical difference between BF group and HD group （t=1. 931, P〉0.05）. The levels IL-17 and IL-23 were （12.1±3.7） ng/L and （84.4±46.0） ng/L respectively in CE group, which were lower than those in BF group [（15.5±4.1） ng/L and （138.6±37. 9） ng/L, respectively; t=2. 515 and 3. 649 respectively, both P〈0.05] and those in HD group [（14.8±4.4） ng/L and （138.1±48. 7） ng/L, respectively; t=2. 401 and 3. 706 respectively,both P 〈0.05], while there was no statistical difference between BF group and HD group （t=0. 534,P 〉0.05）. Serum concentrations of IL-17 were all positively correlated with the concentrations of IL23 in these three groups （r=0. 657, P〈0.05）. Conclusion The frequeny of Th17 cells in PBMC and the serum concentrations of IL-17, IL-23 are significantly reduced in patients with cystic echinococcosis.