上海地区2009年人群甲型流行性感冒病毒抗体水平检测分析

Analysis of levels of antibodies against influenza A virus of population in Shanghai during 2009

目的 了解上海地区人群季节性H1、H3亚型流感病毒抗体水平及职业人群中H5、H9亚型禽流感抗体的检出情况.方法 应用血凝抑制试验对上海地区2009年与禽类密切接触的职业人群356人以及一般人群332人各年龄组进行H1、H3、H5、H9亚型流感病毒抗体的血清学监测.结果 A/Brisbane/59/2007（H1N1）抗体阳性分布,一般人群有275人,占82.8%,职业人群有263人,占73.9%;A/Brisbane/10/2007（H3N2）抗体阳性,一般人群有168人,占50.6%,职业人群有195人,占54.8%;人群中A/Brisbane/59/2007（H1N1）抗体阳性率明显高于H3,与2008年上海地区流感病原学监测情况相吻合.职业人群与一般人群H5亚型抗体阳性率分别为4.2%（15/356）和0.3%（1/332）;H9亚型抗体阳性率分别为34.6%（123/356）和2.4%（8/332）.不同年龄组中,6个月～5岁年龄组和≥60岁的老年组A/Brisbane/59/2007（H1N1）、A/Brisbane/10/2007（H3N2）抗体阳性率较低.结论 上海地区人群对H1、H3季节性流感有较强的免疫保护,儿童和老年人群H1、H3季节性流感抗体水平较低.职业人群禽流感病毒H5、H9抗体有明显升高趋势,要加强对职业人群流感样患者病原学和血清流行病学监测.

Objective To know the levels of antibodies against influenza A virus subtypes H1 and H3 of population in Shanghai during 2009, and the detection of antibodies against avian influenza virus subtypes H5 and H9 in population which contacts with avian. Methods The serological survey of the antibodies against influenza A viruses subtypes H1, H3, H5 and H9 in 356 close contacts with avian （professional population） and 332 general subjects （general population） at various age groups were carried out using hemagglutinin inhibit （HI） test. Results The positive rates of antibodies against influenza virus A/Brisbane/59/2007 （H1N1） in general population and professional population were 82.8% （275/332） and 73.9% （263/356）, respectively; those of A/Brisbane/10/2007 （H3N2）were 50.6% （168/332） and 54.8% （195/356）, respectively. The positive rate of antibodies against influenza virus A/Brisbane/59/2007 （H1N1 ）was significantly higher than that of influenza A viruses subtype H3, which was consistent with etiological survey of influenza virus in Shanghai during 2008.The positive rates of antibodies against influenza A virus subtype H5 in professional population and general population were 4.2% （15/356） and 0.3% （1/332）, respectively; those of influenza A virus subtype H9 were 34.6% （123/356） and 2.4% （8/332）, respectively. The positive rates of antibodies against influenza virus A/Brisbane/59/2007 （H1N1 ） and A/Brisbane/10/2007 （H3N2） in age groups of 6 months-5 years and ≥60 years were lower than other age groups. Conclusions The immune protective response against seasonal influenza A subtype H1 and H3 of population in Shanghai is high,while those of children and the elders were low. The levels of antibodies against influenza A viruses subtype H5 and H9 in professinal population present obviously ascending trend, which indicates that the etiological and serological survey of influenza virus in this population should be enhanced.