云芝菌株遗传多样性的ISSR分析(英文)

Genetic diversity of Trametes versicolor revealed by inter-simple sequence repeat markers

来自全国的34株野生菌株经形态学特征和结合ITS序列鉴定为云芝。采用ISSR标记技术对34株野生云芝菌株进行遗传多样性分析。从20条引物中筛选出7条ISSR引物,扩增得到95个扩增位点,其中多态性位点88个。多态性位点占92.6%,表明ISSR标记的多态性非常高。基于ISSR条带构建亲缘关系树状图,其中遗传变异系数范围为0.58-0.91。34个云芝菌株在相似系数0.60时分为4个类群,不同菌株的遗传差异性与地理分布有一定联系。

Thrity-four wild strains isolated from China were identified as Trametes versicolor based on morphological characteristics and molecular taxonomic evidence.The molecular classification of T.versicolor is highly consistent with the traditional classification.The genetic diversity of these strains was evaluated by analysis of inter-simple sequence repeat（ISSR）.Ninty-five countable bands were produced by 7 primers,of which 88（92.6%） bands were polymorphic.ISSR markers clearly discriminated the differences among tested strains.The dendrogram based on ISSR data was constructed with similarity coefficients ranging from 0.58 to 0.91 and the strains were divided into 4 groups at a level of 0.60 similarity coefficient.Genetic diversity of different strains was related to geographic origins.