盐胁迫下构树DREB转录因子基因表达的实时荧光定量PCR分析

DREB Gene Expression in Leaves of Broussonetia papyrifera Seedlings under Salt Stress Detected by Real-Time Fluorescent Quantitative PCR

转录因子是指那些专一性地结合于DNA特定序列上、能激活或抑制其他基因转录的蛋白质（王少峡等,2004）。DREB（dehydration responsive element binding protein）转录因子是一种特异性的转录因子,当植物在干旱、低温及盐等逆境胁迫下,

Real-time fluorescent quantitative PCR was used to datect DREB genes＇ expression levels in leaves of Broussonetia papyrifera seedlings at 0,6,12 and 24 h after treatments with 100,200 and 300 mmol·L -1 NaCl. The results showed that BpDREB1 and BpDREB2 genes were induced by 100 and 200 mmol·L -1 NaCl to increase their expression levels. But the levels decreased at later stage by the salt stress. Treatment with 300 mmol·L -1 NaCl caused reduced expression levels of the two genes. The experiment verified that the transcriptional expression of DREB genes in B. papyrifera was able to be induced by salt stress,which might be involved in signalling pathways in response to the salt stress. But the genes were prohibited by higher salt concentration or longer duration of NaCl stress.