依赖纤维蛋白单体的细胞伸展及其意义

Fibrinmonomer Dependent Cell Spreading and its SignificanceYang Wei, Asakura Shinji, Matsuda Michio

目的：检测肿瘤细胞表面纤维蛋白受体的分布情况及整合素相关蛋白（ｉｎｔｅｇｒｉｎａｓｓｏｃｉａｔｅｄｐｒｏｔｅｉｎ，ＩＡＰ）参与细胞伸展的状况。方法：采用免疫组织化学法观察纤维蛋白单体做为配体时人神经胶质瘤细胞表面受体的分布情况；并采用细胞粘附及抑制方法观察ＩＡＰ与纤维蛋白单体引起的细胞伸展的关系。结果：纤维蛋白单体不仅能识别体外连接蛋白受体（ｖｉｔｒｏｎｅｃｔｉｎｒｅｃｅｐｔｏｒ，αｖβ３），而且能识别纤维连接蛋白受体（ｆｉｂｒｏｎｅｃｔｉｎｒｅｃｅｐｔｏｒ，α５β１）；纤维蛋白单体介导的细胞伸展不能被抗ＩＡＰ抗体所抑制。结论：纤维蛋白单体可能动员两个或两个以上的整合素引起细胞仲展。α５β１可能较αｖβ３更为重要。

Objective: Our aim was to study the distribution profiles of the fibrin receptors on tumor cells and the relationship of integrin associted protein(IAP) with fibrinmonomer dependent cell spreading. T5HZMethods: Using immunohistochemical staining technique, we studied the distribution profiles of receptor on human glioma cells with fibrinmonomer as ligand. By cell adhesion assay and adhesion inhibition assay, we studied the relationship of IAP and cell spreading induced by fibrinmonomer. Results: Fibrinmonomer recognized and showed the affinity for not only vitronectin receptor(v3) but also fibronectin receptor(51). Cell spreading induced by fibrinmonomer could not be inhibited by antiIAP monoclonal antibody. Conclusion: Two or more than two integrins might be mobilized fibrinmonomer to make cells spreading.51 might play a pivotal role in fibrinmonomer dependent cell spreading.