摘要
目的:探讨黄芪对单线态氧的作用。方法:应用兔离体心肌缺血再灌注损伤模型,将心脏随机分成对照组、缺血组和黄芪组(每组5只)。对照组心脏不给予缺血,只给予有氧灌注40min;另两组心脏在常温下(37℃)给予30min缺血和10min再灌注;黄芪组在Thomas液中加入黄芪注射液(5ml/100ml)。实验过程中检测心肌组织中的MDA以及外部-5′-核苷酸酶的活性。结果:黄芪能有效地保护外部-5′-核苷酸酶的活性,使MDA含量明显减少(P<0.05)。结论:在缺血心肌再灌注过程中,黄芪可能具有清除单线态氧的作用。
Objective: Our aim was to
study the scavenging effect of Astragslus membranaceus on singlet oxygen during ischemia
and reperfusion for the isolated rabbit heart. Methods: Applying the experimental models of
isolated rabbit myocardial ischemia/reperfusion,we rondomly assigned the isdated rabbit
hearts into three groups (n=5/group). The ecto5nucleotidase activity and MDA in myocardial
tissue were measured. WT5HZResults: Astragali injection could effectively protect
ecto5nucleotidase activity(P<0.05) and largely reduced the amount of MDA (P<0.05).
Conclusion: During the course of ischemia and reperfusion,Astagalus membranaceus might
have the ability of scavenging singlet oxygen.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
1999年第3期184-185,共2页
Journal of China Medical University
关键词
黄芪
核苷酸酶
单纯态氧
心肌缺血
再灌注损伤
singlet oxygen
astagalus
membranaceus
myocardial reperfusion injury
5nucleotidase