摘要
目的：探讨黄芪对单线态氧的作用。方法：应用兔离体心肌缺血再灌注损伤模型，将心脏随机分成对照组、缺血组和黄芪组（每组５只）。对照组心脏不给予缺血，只给予有氧灌注４０ｍｉｎ；另两组心脏在常温下（３７℃）给予３０ｍｉｎ缺血和１０ｍｉｎ再灌注；黄芪组在Ｔｈｏｍａｓ液中加入黄芪注射液（５ｍｌ／１００ｍｌ）。实验过程中检测心肌组织中的ＭＤＡ以及外部－５′－核苷酸酶的活性。结果：黄芪能有效地保护外部－５′－核苷酸酶的活性，使ＭＤＡ含量明显减少（Ｐ＜０．０５）。结论：在缺血心肌再灌注过程中，黄芪可能具有清除单线态氧的作用。
Objective: Our aim was to
study the scavenging effect of Astragslus membranaceus on singlet oxygen during ischemia
and reperfusion for the isolated rabbit heart. Methods: Applying the experimental models of
isolated rabbit myocardial ischemia/reperfusion,we rondomly assigned the isdated rabbit
hearts into three groups (n=5/group). The ecto5nucleotidase activity and MDA in myocardial
tissue were measured. WT5HZResults: Astragali injection could effectively protect
ecto5nucleotidase activity(P<0.05) and largely reduced the amount of MDA (P<0.05).
Conclusion: During the course of ischemia and reperfusion,Astagalus membranaceus might
have the ability of scavenging singlet oxygen.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
1999年第3期184-185,共2页
Journal of China Medical University
关键词
黄芪
核苷酸酶
单纯态氧
心肌缺血
再灌注损伤
singlet oxygen
astagalus
membranaceus
myocardial reperfusion injury
5nucleotidase
