ACE基因I/D多态性与系统性红斑狼疮肾脏易感性关系的研究

Study of relationship between ACE gene I/D polymorphism and renal predisposition in SLE

目的研究血管紧张素转换酶（ＡＣＥ）基因Ｉ／Ｄ多态性与系统性红斑狼疮（ＳＬＥ）肾脏易感性的关系。方法用ＰＣＲ法和比色法分别测定５８例ＳＬＥ患者和４０例正常人的ＡＣＥ基因型和血清ＡＣＥ活性。结果确诊ＳＬＥ时临床有肾脏受累表现组ＡＣＥ基因ＤＤ型和Ｄ等位基因分布频率明显高于对照组，且ＤＤ型、ＤＩ型者血清ＡＣＥ活性前者明显高于后者。结论ＡＣＥ基因的Ｄ等位基因可能是ＳＬＥ肾脏易感性的一个危险因子，ＡＣＥ基因型和ＡＣＥ活性水平可作为推测ＳＬＥ肾脏易感性的参考指标。

Objective To investigate the relatioship between angiotensin converting enzyme (ACE) gene I/D polymorphism and renal predisposition in patients with SLE. Method ACE genotype and serum ACE activity in 58 cases of SLE and 40 normal controls were determined by PCR and spectrophotometic assay then analysied with correlative clinical dat. Results We found that ACE gene DD genotype and D allele frequences were higher in SLE samples with clinical lesion than in the controls,and the former with DD and DI genotype had higher serum ACE activity than the latter. Conclusion D allele of ACE gene was a possible risk factor of suseptibitity of renal lesion in the patients with SLE.ACE genotype and ACE activity level may be a reference index to speculate renal predisposition in SLE patients.