摘要
近年来分子生物学和遗传学的迅速发展,使人们对基因结构与功能的认识日益加深。科学工作者们发现基因的某些突变可导致疾病的发生或易感性增加。因此可以通过对疾病相关基因的分析进行疾病诊断或预告;对于那些尚未确定致病基因的遗传病也可利用DNA标记进行遗传连锁分析?..
Capillary electrophoresis (CE ), with minute quantities of sample running in a narrowbore(50~100μm i. d. ) capillary of fused silica tubing, has become an attractive alternative of classic slab gel electrophoresis by its fast separation, high sensitivity, and automated operation. This review introduces the coupling of CE with PCR for gene diagnosis of a series of genetic diseases and qualitative or quantitative analysis of infectious diseases. It also opens up broad prospects for detection of susceptibility factors for common diseases. Furthermore, the authors describe the use of CE in studies on mechanism of tumor pathogenesis and monitoring of the therapeutic effect of tumor treatment. Further applications of this technique are now being studied.
出处
《基础医学与临床》
CSCD
1999年第3期94-96,共3页
Basic and Clinical Medicine