小檗碱对EB病毒DNA复制的抑制作用

Inhibitory effect of berberine on Epstein-Barr DNA replication

目的通过分析小檗碱处理后的Raji细胞中EB病毒DNA的变化,探讨小檗碱对EB病毒DNA复制的抑制作用。方法运用四甲基偶氮唑蓝法确定小檗碱对Raji细胞生长的细胞无毒性浓度,然后采用巢式荧光定量PCR方法检测该浓度处理后的Raji细胞EB病毒DNA含量,分析小檗碱对EB病毒DNA复制的影响。结果小檗碱对Raji细胞的细胞无毒性浓度(NCC)为0～10μmol/L.不同浓度(1.25、10和40μmol/L)的小檗碱处理后的Raji细胞EB病毒DNA拷贝数与未加药组相比显著降低(P<0.05),呈药物浓度依赖性。结论小檗碱在细胞无毒性浓度下,可抑制Raji细胞中EB病毒DNA的复制,为使用小檗碱或含小檗碱的中药复方防治EB病毒相关肿瘤提供了新的实验依据。

ObjectiveTo observe the effect of berberine （BBR） on EBV-DNA replication in Raji cells treated with BBR.MethodsMTT assay was used for determining non-cytotoxic concentration （NCC） of BBR in Raji cells. After being treated with BBR at NCC, EBV-DNA was detected by fluorescent quantitative nested-PCR, and then the inhibitory effect of BBR was analyzed on EBV-DNA replication in Raji cells at various concentrations.Results NCC of BBR in Raji cells was 0～10 μmol/L, EBV-DNA levels of Raji cells with BBR treatment at 1.25, 10 and 40μmol/L were obviously lower than that without using BBR （P 0.05）.ConclusionBBR could inhibit EBV-DNA replication at NCC, it provides a new experimental evidence to prevent and cure nasopharyngeal carcinoma using BBR or the traditional Chinese drug compound recipes comprised of BBR.