急性胰腺炎患者血浆对氧磷酯酶1活性的测定及临床意义

Plasma paraoxonase 1 activity in patients with acute pancreatitis and its clinical significance

目的探讨急性胰腺炎患者血浆对氧磷酯酶1(PON1)活性变化及其与急性胰腺炎之间的关系。方法检测53例急性胰腺炎患者和46例健康对照者血浆对氧磷酯酶1及超氧化物歧化酶(SOD)活性、丙二醛(MDA)和血脂水平,并进行相关性分析。结果急性胰腺炎组血浆对氧磷酯酶1和超氧化物歧化酶活性分别为(132.2±64.3)kU/L和(79.2±10.2)U/mL,均较对照组显著降低(168.3±72.1)kU/L和(91.2±11.1)U/ml,P<0.05。丙二醛含量较对照组显著升高([14.4±3.8)μmol/Lvs(10.1±3.1)μmol/L,P<0.01]。重度胰腺炎组PON1和SOD活性显著低于轻度组(P<0.05),而MDA水平显著高于轻度组(P<0.05)。对氧磷酯酶1与超氧化物歧化酶活性呈正相关(r=0.411,P<0.05),与丙二醛水平呈负相关(r=-0.498,P<0.01)。结论急性胰腺炎患者血浆对氧磷酯酶1活性显著降低,可能与氧自由基的增加有关。降低的PON1活性可能参与了急性胰腺炎的病理生理过程。

【Objective】To investigate the change of plasma paraoxonase 1 activity （PON1） in the patients with acute pancreatitis and the relationship between paraoxonase 1 activity and acute pancreatitis. 【Methods】Plasma paraoxonase 1 activity, superoxide dismutase （SOD） activity, malondialdehyde （MDA） levels and lipids levels in 46 normal controls and 53 acute pancreatitis patients were determined. The correlation analysis was completed.【Results】In acute pancreatitis patients plasma PON1, MDA and SOD were （132.2±64.3） kU/L, （14.4±3.8） μmol/L and （79.2±10.2） U/mL, respectively. In the normal control subjects PON-1, MDA and SOD were （168.3±72.1） kU/L, （10.1± 3.1） μmol/L and （91.2±11.1） U/ml, respectively. Compared with the control group, the activity of both PON1 and SOD was markedly decreased （P 0.05）, MDA level was increased in the patients with acute pancreatitis （P 0.05）. Among the patients with acute pancreatitis, there were lower plasma activity of PON1 and SOD, and higher MDA level in patients with severe pancreatitis than in those with mild pancreatitis （P 0.05）. PON1 activity was positively associated with SOD activity （r =0.411, P 0.05）, was negatively associated with MDA level （r =-0.498, P 0.05）.【Conclusion】 PON1 activity is lower in the patients with acute pancreatitis which maybe result from increased oxygen free radical levels. Decreased PON1 activity may have a role in the pathogenesis of acute pancreatitis.