静电纺PLGA/姜黄素复合薄膜的制备及抗凝血研究

Preparation and Anticoagulation Evaluation of Electrospun PLGA/Curcumin Composite Films

为了研究姜黄素的抗凝血性能，采用静电纺丝技术，以生物可降解PLGA为载体制备了PLGA与姜黄素质量比分别为4：1、2：1和3：2的纳米纤维复合薄膜。采用傅里叶变换红外光谱研究了复合薄膜的组成成分，结果显示姜黄素与PLGA的特征峰均出现在复合薄膜的红外图谱中；利用扫描电镜（SEM）及相关分析软件分析了不同质量比时纳米纤维膜的表面形态及直径分布。在实验范围内，复合纳米纤维的平均直径为370-800nm，均匀性较好，且纤维平均直径随姜黄素含量的增加而增大。同时研究了复合薄膜的部分凝血活酶时间（APTT），结果表明，复合薄膜的APTT比原血浆的延长了18s，说明PIGA／姜黄素复合薄膜具有良好的抗凝血性能，且抗凝血性能随姜黄素含量的增加而进一步提高。

In order to study the anticoagulation properties of curcumin, different mass ratios（4 ： 1,2 ： 1 and 3 ：2 of PLGA and curcumin） composite films are prepared using a biodegradable polyrner-PLGA as the carrier of curcumin by electrostatic spinning technology. The component film is analyzed by fourier transform infrared spectroscopy （FTIR）, and the major peaks of curcumin and PLGA are both observed in the composite film The diameter-distribution and morphology of different nanofibres are analyzed by scan electric microscope（SEM） and related software. Within the framework of the experiment, the average diameter of composite nanofibers is between about 370nm and 800nm with better uniformity, furthermore the average diameter of composite nanofibers increases with the curcumin content increase At the same time, activated partial thromboplastin time（APTT） of all composite films are analysed. The results indicate that all composite films have better anticoagulation effect which is lengthened by 18s, compared with the original plasma. In addition, all anticoagulation tests indicate the higher the curcumin content is in the film, the better the antieoagulation effect is.