碳纤维/聚乳酸/壳聚糖三元多孔复合支架材料的制备及评价

Preparation and Evaluation of Porous 3D CF/PLA/CS Composites Scaffold

利用溶液共混法以及冷冻干燥法制备了多孔碳纤维／聚乳酸／壳聚糖（CF／PLA／CS）三元复合生物材料，通过扫描电子显微镜（SEM）观察了其表面形貌特征，并对其细胞相容性进行了评价。实验结果表明，利用上述复合方法制备的三维多孔材料的孔径为20-500μm，孔分布均匀，孔与孔之间相互连通；碳纤维的分散、冷冻干燥温度对材料结构影响较大，随冷冻温度的降低，支架的孔隙变小、变规则，内部结构趋向均一，孔隙率有所降低；该材料与细胞具有较好的相容性，符合组织支架材料的基本要求。

The porous CF/PLA/CS composite scaffold is prepared by using solvent blending process and freezedrying technique. The surface morphology is observed by using SEM, and the cell compatibility is also evaluted. The results show that the size of pores in the composites are 20-500μm, the pores are well distributed, and connected with each other. The decentralize of CF and the temperature of freeze-drying play an important role in control the structure of the composites, and the temperature is lower, the pore is smaller, and more orderly, but the porosity decreases. The cell test shows that the cell compatibility of composites is excellent, which can meet to the requires of tissue engineering scaffold.