HPV-DNA分型检测在宫颈病变筛查中的应用价值

Value of human papillomavirus genotype testing in screening of cervical lesions

目的评价HPV-DNA分型检测在宫颈病变筛查中的应用价值。方法对200例细胞学筛查阳性病例进行阴道镜下多点取材活检及HPV分型检测。结果 HPV阳性121例,阳性率为60.50%;感染频度前5位依次为16、58、39、6、33型。宫颈细胞学检查异常HPV阳性确诊为CIN者73例,HPV阴性确诊CIN者13例,两者有统计学差异(χ2=50.96%,P<0.05)。细胞学诊断为ASC的病例中,HPV阳性组宫颈病变检出率(19/68)与阴性组(5/75)对比,两者有统计学差异(χ2=14.01,P<0.05)。结论 HPV分型检测对宫颈病变具有很高的敏感度和阴性预测值,在宫颈病变筛查及HPV疫苗研发中具有重要意义。

【Objective】To assess the value of human papillomavirus（HPV） genotype testing in screening of cervical lesions.【Methods】Two hundred cases of atypical squamous cells（ASC） or more severe than ASC lesions are classified into positive cytology.These cases were referred for biopsy and HPV genotype testing.【Results】Positive HPV testing appeared in 121 cases,positive rate was 60.50 %.The top five prevalent HPV types were type 16,58,39,6 and 33.Among these cases with definite diagnosis of CIN by biopsy,73 cases showed positive HPV testing,13 cases were HPV negative with statistic difference（χ2 =50.96%,P 0.05）.Among these cases of atypical squamous cells（ASC）,the rates of CIN in the group of positive HPV testing and in the group of negative were different in statistics（χ2 = 4.01,P 0.05）.【Conclusion】HPV genotype testing has high sensitivity and negative-predictive value,and provides very useful information for the screening of cervical lesions and development of HPV vaccine.