肝细胞癌中podoplanin的表达及其与淋巴道转移关系的研究

Investigation of relation between expression of podoplanin and lymphatic metastasis in human hepatocellular carcinoma

目的观察肝细胞癌组织淋巴管内皮细胞podoplanin的表达,为研究肝细胞癌淋巴道转移的分子机制提供形态学依据。方法用SP免疫组化检测法对肝细胞癌组织、淋巴结及正常肝组织中podoplanin的表达情况进行观察;用podoplanin来标记淋巴管,检测癌组织中微淋巴管密度。结果肝细胞癌组织中血管内皮细胞podoplanin不染色,淋巴管内皮细胞podoplanin阳性表达。经计数微淋巴管数量,癌组织和癌旁肝组织微淋巴管密度明显高于正常肝组织(P<0.01)。结论podoplanin可选择性的在肝细胞癌组织淋巴管内皮表达,能明确的区分血管及淋巴管成分,可作为一个淋巴管内皮细胞较特异的标记物。由于微淋巴管密度在癌组织中的表达明显增高,说明淋巴管数量的增加可能与肝细胞癌的淋巴转移有关。

【Objective】To observe the expression of podoplanin in lymphatic endothelial cells in the human hepatocellular carcinoma（HCC） so as to provide morphological data for the study on the molecular mechanism of tumor metastasis,.【Methods】The expression of podoplanin in hepatocellular carcinoma,lymph nodes and normal liver tissue was observed by SP immunohistochemistry.Using podoplanin as the specific marker of lymphatic endothelium,lymphatic micro-vessel density（LMVD） in hepatocellular carcinoma was calculated.【Results】 Podoplanin was not stained in blood vessels,but podoplanin was positively expressed in the human HCC lymphatics.LMVD of HCC tissue and pericancerous liver tissue was obviously higher than that in normal hepatic tissues（P0.05）.【Conclusions】Podoplanin is selectively expressed in lymphatics of HCC and could correctly distinguish blood vessels from lymphatic vessels.It could be regarded as the specific marker of lymphatic vessels.The positive rate of podoplanin expression increases in HCC and metastatic lymph nodes,therefore,the increased count of lymphatic vessels likely contributes to lymphatic spread of HCC.