摘要
建立检测谷物中脱氧雪腐镰刀菌烯醇（DON）的液相色谱方法，通过不同提取方法的比对来确定最佳的DON提取方案，并最终确定使用乙腈－水（体积比V/V，84／16）提取，经过DON专用净化柱的净化处理后，采用紫外检测器（λ=220nm）CLC18亲水分离柱测定。结果表明，用高速均质机均质4min提取，可大幅度提高方法回收率，回收率达到90．7％－98．9％，方法最低检出限为50μg／kg。
A HPLC method was developed for deoxynivalenol （DON） in grains. Extraction was carried out with aceronitrile-water mixture （84/16,V/V）, followed by a clean-up step with TRILOGY columns. The extracts was analyzed by UV detector （λ = 220 nm） and C18 column. The results showed that using high-speed Homogenizer extracts 4 min, greatly increased the recovery rate（90.3%-102.1%）,the lowest detection limits was 50 μg/kg.
出处
《粮食加工》
2010年第6期92-94,共3页
Grain Processing
