新疆哈萨克族儿童基因rs9930506多态性与超重、肥胖的关联

The research of association between gene rs9930506 polymorphism and Hazakh children with overweight or obesity in Xinjiang

目的 研究新疆哈萨克族学龄儿童体脂量和肥胖症相关基因（FTO）rs9930506与超重、肥胖的关系.方法 2009年5-6月采用整群抽样法在新疆伊犁哈萨克自治州选取118名超重、肥胖儿童作为研究对象,对照组为141名体重正常的儿童.应用聚合酶链反应-限制性片段长度多态性技术分析2组儿童rs9930506位点多态性,同时使用自动生化仪酶法检测血糖、血脂,放射免疫法检测胰岛素浓度.结果 rs9930506在超重、肥胖组和对照组中基因型频率分别为：AA型0.458、0.582;AG型0.492、0.397;GG型0.050、0.021.两组AA、AG和GG基因型频率分布差异无统计学意义（x2=4.795,P=0.091）,但GG＋AG基因型频率在超重、肥胖组（0.542）高于对照组（0.418）,差异有统计学意义（x2=3.957,P=0.047）;A等位基因频率在超重、肥胖组和对照组分别是0.703、0.780,差异有统计学意义（x2=3.986,P=0.046）.超重、肥胖组中GG;＋AG基因型人群及AA基因型者的空腹血糖水平分别是（4.93±0.52）、（4.69±0.56）mol/L（t=2.430,P=0.017）,臀围分别是（79.70±11.73）、（75.28±11.52）cm（t=2.053,P=0.042）,体质指数（BMI）分别是（21.61±2.72）、（19.92±1.98）kg/m2（t=3.891,P=0.000）,GG＋AG基因型人群空腹血糖水平（t=2.430,P=0.017）、臀围（t=2.053,P=0.042）、BMI（t=3.891,P=0.000）均高于AA基因型者.结论 FTO基因rs9930506多态性和新疆哈萨克族学龄儿童超重、肥胖相关,与空腹血糖水平、臀围、BMI差异具有相关性.

Objective To study the association of fat mass and obesity associated gene rs9930506 polymorphism with overweight and obesity in the Hazakh Children. Methods PCR-restriction fragment length polymorphism was used to determine rs9930506 polymorphism in 118 patients with overweight or obesity and 141 controls. Serum lipid level including total cholesterol, triglyceride, high-density and lowdensity lipoprotein cholesterol, plasma glucose levels, and plasma insulin were monitored by using enzymatic method（Hitachi automatic clinical analyzer） and radioimmunoassay kit respectively. Results The genotype distributions of the control group and overweight or obesity group were in the Hardy-Weinberg equilibrium. The frequencies of AA,AG and GG were 0. 458,0. 492 and 0. 050 in the overweight or obesity group and 0. 582,0. 397 and 0. 021 in the controls respectively. There was no significant difference in frequencies of AA,AG and GG genotypes between control group and overweight or obesity group（x2 =4. 795,P =0. 091）, but the frequency of GG ＋ AG genotype in overweight or obesity group（0. 542） was higher than that in the controls （0. 418） giving significant difference （x2 = 3. 957, P = 0. 047） . The frequencies of A allele were 0. 703 in the overweight or obesity group and 0. 780 in the controls respectively. There was significant difference in frequencies of A allele frequency between controls and overweight or obesity group（x2 =3. 986,P =0. 046）. In the overweight or obesity group,the plasma glucose levels, BMI and hip circumference were （4. 93 ± 0. 52） mol/L, （79. 70 ± 11.73） and （21.61 ± 2. 72） cm in the persons with GG ＋ AG genotype and （4. 69 ±0. 56） mol/L, （75.28 ± 11.52） and （19. 92 ± 1.98） cm in those of AA genotypes respectively. The plasma glucose levels,BMI and hip circumference of the persons with GG ＋ AG genotype were higher than those of AA genotypes and the statistical significance was also observed （t=2.430,P=0. 017;t=2.053,P=0. 042;t=3.891,P=0. 000）.Conclusion The present findings suggest that rs9930506 polymorphism on chromosome 16q12. 2 may be associated with the development of overweight or obesity in the Hazakh Children, and the polymorphism may have some influence on serum glucose levels, BMI and hip circumference in this population.