摘要
Proteomics was used to reveal the differential protein expression profiles of acute responses to copper sulfate exposure in larvae of Artemia sinica.Fourteen differentially displayed protein spots were detected and seven of them were identified.Three spots were up-expressed and identified:actin, heat shock protein 70,and chaperone subunit 1;three down-regulated proteins were identified:arginine kinase,elongation factor-2,and glycine-rich protein;and a newly expressed protein was identified as peroxiredoxin.The study indicates the involvement of all the differentially expressed proteins in the early responses of protein expression,and in the survival of A.sinica in the presence of copper and other heavy metals;the findings improve understanding of the organism’s adaptive responses and resistance.
Proteomics was used to reveal the differential protein expression profiles of acute responses to copper sulfate exposure in larvae of Artemia sinica. Fourteen differentially displayed protein spots were detected and seven of them were identified. Three spots were up-expressed and identified： actin, heat shock protein 70, and chaperone subunit 1; three down-regulated proteins were identified： arginine kinase, elongation factor-2, and glycine-rich protein; and a newly expressed protein was identified as peroxiredoxin. The study indicates the involvement of all the differentially expressed proteins in the early responses of protein expression, and in the survival ofA. sinica in the presence of copper and other heavy metals; the findings improve understanding of the organism＇s adaptive responses and resistance.
基金
Supported by the Natural Science Foundation of China(No.20060109Z4016)
the National Basic Research Development Program of China(No.2006CB101804)
the Natural Science Foundation of Shandong Provincefor the excellent young researchers(No.2006BSA02004)
