时间分辨免疫荧光与ELISA法检测乙型肝炎病毒血清标志物结果的比较与分析

An analysis and comparison on the detection of Hepatitis B virus serum markers between time-resolved immunofluorescence and ELISA

目的：探讨时间分辨免疫荧光技术（TRFIA）定量检测乙型肝炎病毒标志物（HBV-M）在临床中的应用情况,并进行TRFIA的方法学研究.方法：实验用TRFIA法与ELISA法分别对200例血清标本进行HBsAg检测,对另200例血清样本进行＂乙肝两对半＂检测,同时检测20份HBsAg低值（弱阳性）标准品血清,并对TRFIA法与ELISA法进行结果比较与分析.结果：TRFIA法HBsAg灵敏度（0.2ng/ml）高于ELISA法,TRFIA法HBsAg线性检测范围在0.2-200ng/ml,且TRFIA法的特异性优于ELISA法,ELISA法在检测0.5-1ng/ml时易出现假阴性;且ELISA法易出现HBsAg、HBeAg或HBeAb假阴性,HBcAb假阳性,导致其他模式增多.结论：TRFIA法具有测量范围宽,灵敏度高、特异性强等优点,在临床检测HBV中具有广泛的应用前景.

Objective： To investigate the clinical application of time-resolved immunofluorescence （TRF1A） used in HBV-M ration test and do some research on TRFIA method. Methods： To do HBsAg test 200 cases of serum samples HBsAg by TRFIA and ELISA and another 200 tested by HBV-M separately, at the same time 20 cases of standard blood in which HBsAg was tested and analyzes by ELISA and TRFIA. Results： TRFIA has higher sensitivity （0.2ng/ml） than ELISA. The specificity of TRFIA is superior to ELISA within the scope of linear test （0.2-200ng/ml）, ELISA shows fake negative in testing in HBsAg, HBeAg or HBeAb, and fakes positive in HBcAb, and make other patterns increasing. Conclusion ：TRFIA has higher specificity and sensitivity than ELISA.