感冒清热口服液中葛根素含量测定

Determination of Puerarin in Ganmaoqingre Oral Liquid by HPLC

目的:应用HPLC法测定感冒清热口服液中葛根素含量。方法:采用高效液相色谱法;色谱柱:ShimpackVP-ODS柱(150mm×4.6mm);流动相:甲醇-水(24:76)流速:1.0ml/min;检测波长:250nm;进样量:20μl。结果:在进样量0.5μg～4μg范围内,葛根素峰面积与进样量呈良好的线性关系,其回归方程为:A=48895170.86C+4178.18;r=0.999996,平均回收率为98.98%,RSD=0.55%。结论:本法快速简便,准确,重现性好。

Objective：HPLC determination of puerarin in Ganmaoqingre oral liquid.Methods：Using high performance liquid chromatography;Column：Shim - pack VP - ODS column（150mm×4.6mm）;The mobile phase：methanol - water （24：76）flow rate：1.0ml/min;detection wavelength：250nm;injection volume;20μl.Results：in the sample volume 0.5μg～4μg within the puerarin peak area and sample volume showed good linear relationship between the regression equation is：A = 48895170.86C ＋4178.18;r = 0.999996 The average recovery was 98.98%RSD = 0.55%.Conclusion：The method is simple,rapid,accurate and reproducible.