超声波辅助提取穿山龙中薯蓣皂苷元的实验研究

Study on the Method of Ultrasonic-Assisted Extracting Diosgenin in Dioscorea Nipponica

目的:研究一种测定穿山龙中薯蓣皂苷元时快速提取薯蓣皂苷元的新方法——超声辅助水解原位萃取法。方法:对影响提取率的重要因素进行了考察,以期建立最佳提取条件。结果:样品在料液比为1:15(g:mL)条件下,用浓硫酸-异丙醇-水(8:22:70)溶液在90℃下超声辅助回流2h,获得最佳的提取率。结论:超声辅助水解原位萃取法省时、操作简便、重现性好。

Objective：To study a new extraction method for analyzing diosgenin in Dioscorea nipponica Makino—extraction in combination hydrolysis in situ.Methods：The effective factors on the yield of diosgenin were evaluated in order to establish the optimum conditions for extraction in combination hydrolysis in situ.Results：Under the condition of the solid -liquid ratio of 1：15（g：mL）,hydrolyses was conducted in sulfuric acid - isopropanol- water（8：22：70）solution at 90℃for 2h with ultrasound - assisted,the best yield of diosgenin was obtained.Conclusion：The method is time - saving,Simple and reproducible.