转基因小麦麦谷蛋白亚基突变体类型

Mutation Types of HMW-GS Component in Transgenic Wheat Progenies

采用十二烷基磺酸钠–聚丙烯酰胺凝胶电泳技术,分析了含外源1Dx5和1Ax1的T7代转基因小麦HMW-GS的突变类型及等位变异.结果显示,随机挑选的1000份B72-8-11bT6代籽粒在T7代中共21类突变类型,其外源1Dx5基因表达频率为49.4%,GluA1、GluB1和GluD1共3、13和9个等位变异类型,分别以N、17+18和N为主,占94.7%、61.5%和49.7%;B102-1-2共11类,外源1Ax1基因表达频率占95%,GluA1、GluB1和GluD1共2、7和3个等位变异类型,分别以1、17+18和N为主,占95%、98.7%和98.3%。研究结果进一步说明了转基因株系的纯合性,完全符合孟德尔遗传分离规律,对小麦优质新品种的培育具有理论意义和应用价值.

Mutation types of HMW-GS component in independent transgenic wheat lines were investigated in this paper.The results showed that there were 21 categories in the 1 000 selected randomly of B72-8-11b,the expression of 1Dx5 gene accounted for 49.4%,three types were detected on Glu A1,and N subunit was the major types with frequency of 94.7%,thirteen types were detected on Glu B1,and 17＋18 subunit was the major types with frequency of 61.5%,nine types were detected on Glu D1,and N subunit was the major types with frequency of 49.7%;There were 11 categories in the B102-1-2,the expression of 1Ax1 gene accounted for 95%,two types were detected on Glu A1,and N subunit was the major types with frequency of 95%,seven types were detected on Glu B1,and 17＋18 subunit was the major types with frequency of 98.7%,three types were detected on Glu D1,and N subunit was the major types with frequency of 98.3%.The results also indicated that the transmission of the transgene and inheritance of its expression followed Mendelian ratios with the method of quantitative genetics,and T7 generation lines almost homozygous.It would be beneficial for deciding the selection strategy in breeding program.