转Bt-cry1Ac基因棉花对意大利蜜蜂肠道细菌群落的影响*

Effect of Transgenic Cotton with cry1Ac Gene on Intestinal Bacterial Community of Apis mellifera ligustica

采用基于16 SrDNA的变性梯度凝胶电泳(DGGE)的方法,研究了转Btcry1Ac基因棉花SGK321对益虫——意大利蜜蜂(Apis mellifera ligustica)肠道细菌群落的影响.分别用转基因/非转基因棉花花粉和高浓度外源DNA饲喂蜜蜂7d,通过DGGE分析经不同处理的蜜蜂肠道细菌群落组成的差异,同时用PCR检测是否发生外源基因的横向转移.结果显示,各个处理组DGGE图谱中的条带数目在11.3～15.0之间,Shannon’s指数在0.87～1.05之间,相似性系数介于68.3%～84.7%,不同处理组肠道细菌组成差异不明显,用PCR的方法也没有检测到外源基因的横向转移.因此初步判定转基因棉花SGK321短期内对蜜蜂细菌群落尚无不利影响.

The effect of transgenic cotton SGK321 with cry1Ac gene on bacterial community structures in honeybee （Apis mellifera ligustica） intestines was studied by denaturing gradient gel electrophoresis （DGGE） based on sequencing of 16S rDNA.The honeybees were fed with the transgenic cotton pollens,non-transgenic cotton pollen and foreign DNA of high concentration for seven days,respectively.The intestinal bacterial diversity of each treatment was analyzed by DGGE and the possibility of a horizontal gene transfer was detected by polymerase chain reaction （PCR）.The DGGE profile showed that the number of bands of all treatments ranged between 11.3～15.0 and Shannon＇s index between 0.87～1.05.The similarity （Dice） coefficient among the treatments ranged from 68.3% to 84.7%.These indices showed that different diets could not change the bacterial diversity in the honeybee intestines.At the same time,gene transfer was not detected.It was concluded that the transgenic cotton had no detrimental effects on the microbial diversity in honeybee intestines in short period of time.Fig 2,Tab 3,Ref 22