骨肉瘤中雌、孕激素受体的表达及意义

Expression and Significance of Estrogen Receptor and Progesterone Receptor in Human Osteosarcoma

探讨骨肉瘤中雌激素受体（ＥＲ），孕激素受体（ＰＲ）的表达及其与骨肉瘤临床特征的关系。应用免疫组化ＳＰ方法（改良ＳＰ法）对６５例骨肉瘤蜡块组织进行了ＥＲ、ＰＲ检测。所有病人术前均未接受内分泌治疗。骨肉瘤中ＥＲ表达的阳性率为４０％（２６／６５），ＰＲ表达的阳性率为３２３％（２１／６５）；阳性颗粒分布于胞浆和胞核；临床无转移，存活时间长的骨肉瘤中ＥＲ，ＰＲ表达的阳性率高于临床有转移，存活时间短者，差异有显著性（Ｐ＜００５）；不同性别、年龄、发病部位的骨肉瘤中ＥＲ、ＰＲ表达的阳性率差异无显著性（Ｐ＞００５）。说明骨肉瘤中存在ＥＲ、ＰＲ，其阳性率的检测为骨肉瘤生物学行为和病人预后的评估及探讨骨肉瘤内分泌治疗提供理论依据。

The expressions of estrogen receptor (ER)、progesterone receptor (PR) in osteosarcoma and the relationship among the ER、the PR and clinical characteristics of the osteosarcoma were studied.The ER and the PR in 65 paraffin embedded examples from the patients with osteosarcoma were detected by using methods of streptavidin/peroxidase (SP) and immunohistochemistry.All patients were not treated with endocrinotherapy before operation.The results showed that the positive rates of the ER and the PR in the osteosarcoma were 40% (26/65),32.3% (21/65) respectively.The positive staining particles were distributed in both cytoplasma and nucleus.The positive rates of expression of the ER and the PR in the sections from osteosarcoma with nonmetastasis and the patients with longer survival time were hight than those in the tumor with opposite conditions.The differences had statistical significance ( P <0 05).The changes of expression of the ER and the PR were not related to sex、age and the tumor site. These findings suggest that the changes of the ER and the PR expressions in the osteosarcoma and their positive rates are important evidences in evaluating the clinical behavior and prognosis of the osteosarcoma.