D-半乳糖胺致急性肝损伤后大鼠胰岛细胞的免疫组织化学研究

Immunohistochemical Changes of Pancreatic Islet Cells following Acute Liver Injury Induced by D-galactosamine in Rats

实验组大鼠腹腔1次注入D-半乳糖胺(600mg/kg)致成急性肝损伤;给予对照组大鼠等量生理盐水。于给药后1、2、3、5、7、10d,禁食12h后分批取胰尾,制石蜡切片。用免疫组织化学PAP法分别显示含胰岛素的B细胞,含高糖素的A细胞和含生长抑素的D细胞。对这些细胞的免疫反应性作半定量观察和分析,以判断肝损伤后胰岛细胞内分泌活动的变化。结果表明,给药后1～5d肝损伤严重,但肝再生也很活跃。此时B细胞胰岛素免疫反应物减少;A细胞高糖素免疫反应物含量明显下降,尤以2、3d最显著;但D细胞的生长抑素免疫反应性则增强。给药后7d肝损伤已基本恢复,3种胰岛细胞的免疫反应性与对照组近似。本研究结果提示,胰岛B细胞和A细胞分泌活动的变化,与肝损伤和肝再生过程密切相关。D细胞的变化可能反映它们对A细胞和B细胞内分泌活动有调控作用。

In this study 71 male Wistar rats were used and divided into experimental group and control group. In the experimental group, D-galactosamine dissolved in physiological saline was given intraperitoneally (600mg/kg). In control group, equal amount of physiological saline was given. After 1, 2, 3, 5, 7 and 10 days, specimens of the tail portion of pancreas were taken under fasted condition for 12 h. The specimens were fixed in Bouin＇s fixative and paraffin sections were made. The immunohistochemical PAP method was used to demonstrate insulin-containing B cells, glucagon-containing A cells and somatostatin-containing D cells. The immunoreactivity of the islet cells was estimated semiquantitatively in order to evaluate the changes of the endocrine activity of the islet cells after liver injury, and the date were analysed statistically, 1～5days after D-galactosamine injection, the liver damage was intensive and regeneration also is active. During the same time, the insulin-immunoreactivity of B ceils decreased; the glucagon-immunoreactivity of A ceils decreased even more, and reached the least at 2nd or 3rd day. On the contrary, the somatostatin-immunoreactivity of D cells increased. At the 7th day, the liver injury tended to recovery and the immunoreactivity of these 3 kinds of islet cells was similar to those of the control group. The results suggest that the changes of endocrine activity of B cell and A cell seem to be closely related to liver injury and regeneration. The changes of D cells may indicate that they exerted probably some regulation roles on the secretion activity of B cell and A cell.