应用FCM检测白血病P^(16)基因产物的表达

Detecting Expression of P 16 Protein of Patients with Leukemia by Flow Cytometry

应用流式细胞术和免疫荧光染色技术对３１例白血病及９例营养性贫血（ＮＤＡ）患者骨髓细胞Ｐ１６蛋白进行检测。结果显示急性淋巴细胞白血病组（ＡＬＬ）Ｐ１６阳性率６３２％，急性非淋巴细胞白血病组（ＡＮＬ）８３３％，营养性贫血组８８９％。ＡＬＬ组Ｐ１６阳性率与ＡＮＬ组相比，差异有显著性（Ｐ＜００１）；与营养性贫血组相比，亦有显著性（Ｐ＜００１）。ＡＮＬ组Ｐ１６阳性率与营养性贫血组相比，差异无显著性（Ｐ＞００５）。提示Ｐ１６基因的改变与淋巴系白血病的发生有密切关系。

The P 16 gene expressions of bone marrow cells in 31 cases of leukemia and 9 cases of nutrition deficiency anemia (NDA) were studied quantitatively by using flow cytometry and immunofluorescence staining technique.The results showed that the positive rates of P 16 protein in ALL,ANL,and NDA were 63 2%,83 3% and 88 9%,respectively.The positive rate of P 16 protein in ALL was significant lower than that in ANL and NDA.There were no remarkable difference in the positive rate of P 16 protein between ANL and NDA.The findings suggest that loss of P 16 is related to the occurrence of ALL.