分式析因设计法对低能离子束介导参数的初步研究

Preliminary Study on the Low Energy Ion Beam Mediated Parameters with Fractional Factorial Design Method

[目的]利用分式析因法探索低能离子束介导各参数的生物学效应。[方法]以同源四倍体双胚苗水稻DER10-04-01为受体材料,以披碱草为DNA供体材料完成离子束介导试验,利用分式析因设计法对低能N+离子束介导外源基因转化水稻的参数进行初步研究。[结果]低能N+离子注入时所采用的能量、剂量、DNA浓渡和DNA浸泡时间等4个因素对DER10-04-01的正常生长发育产生明显的生物学效应,其中注入剂量与DNA浓度对其产生的生物学效应更明显。[结论]能量、剂量、DNA浓渡和DNA浸泡时间是离子束介导转化外源遗传物质中对试验效果具有重要影响的主要因素。

[ Objective ] The aim was to explore the biological effect of low energy ion beam mediated parameters with fractional factorial design method. [ Methodl The twin-embryos seed of autotetraploid rice DER10-04-01 was taken as the receptor material, and the Elymus dahuricus Turcz. was used as materials to provide DNA to carry out the ion beam mediated experiment. And the fractional factorial design method was used to study the parameters of low enery N ＋ ion beam mediated foreign genes into rice. [ Result ] The implantation energy, dose, DNA concentrations and immersion time of DNA showed significant biological effects on the normal growth and development of DER10-04-01, in which the biological effects of implantation dose and DNA concentrations were relatively obvious. [ Conclusion] The implantation energy, dose, DNA concentrations and immersion time of DNA were major factors showing important effects on the experimental result in ion beam mediated foreign genetic materials.