葛仙米提取物诱导肝癌细胞HepG2凋亡的活性研究

Induction of Apoptosis in HepG2 Cells by Nostoc sphaeroides Extract

[目的]研究葛仙米提取物诱导肝癌细胞HepG2凋亡的活性。[方法]采用二甲基四氮唑蓝(MTT)法测定细胞存活率,形态学法观察凋亡的特征性变化,AnnexinV/PI双染及PI单染流式细胞术法检测凋亡率。[结果]经不同浓度提取物作用后,HepG2细胞的生长受到显著抑制,并呈现时间-浓度依赖关系趋势。观察细胞形态,发现有凋亡特征性变化。AnnexinV/PI双染结果显示,癌细胞早期凋亡率随提取物浓度的升高而显著增高,最高可达79.2%。PI法同样显示凋亡的发生,其凋亡峰最大比率为9.6%,显著高于对照组。[结论]葛仙米甲醇提取物能够诱导肝癌细胞HepG2的凋亡。

[Objective]The aim was to investigate the induction of HepG2 cells apoptosis by methanol extractive of Nostoc sphaeroides.[Method]The MTT assay,morphology observation and flow cytometry analysis were used to determine the cell viability,observe the characteristic changes of apoptosis and detect the apoptosis rate.[Result]The results showed that the different contents of extractive could inhibit the viability of HepG2 cells significantly and presented time-concentration dependent relation by MTT assay.The characteristic morphlogical changes of apoptosis were also obtained by observation.The early apoptosis rate was increased clearly with the increasing concentrations of methanol extractive by the AnnexinV/PI binding analysis and the maximum was 79.2%.The maximum rate of apoptosis was 9.6%,which is significantly higher than the contrast by the PI assay.[Conclusion]The methanol extractive of Nostoc sphaeroides was able to trigger HepG2 cells apoptosis.