紫外光诱导硅表面寡聚乙二醇单分子膜的制备

The Preparation of OEG Monolayer on Silicon Surfaces by UV-induced Hydrosilylation

研究报道了一种表面具有抗蛋白吸附性能硅材料的制备方法。该方法利用紫外光线(波长为254 nm)诱导硅氢烷基化反应机理,将寡聚乙二醇分子共价偶联到硅氢化表面上;采用多种表面分析手段(X射线能谱仪、原子力显微镜、椭圆偏振光谱仪、接触角测量仪等)对所得材料进行综合表征,结果显示寡聚乙二醇分子在硅表面上形成了一层致密的单分子膜。最后试验通过测试寡聚乙二醇膜对纤维蛋白原的吸附性能,表征了修饰后硅表面抗蛋白吸附和热稳定性能。结果表明,寡聚乙二醇单分子膜不仅具有良好的抗蛋白吸附性能,而且在生理环境下可以保持长期的稳定性。由此可以推断,这种表面具有抗蛋白吸附性能的硅材料将在药物载体、蛋白检测芯片、生物微机电系统以及生物传感器等研究领域具有一定的应用价值。

A simple method of modification the silicon surfaces with the protein-resistance ability had been reported.Oligo（ethylene glycol）（OEG） terminated monolayer was prepared on hydrogen terminated silicon（111） surfaces by UV-induced hydrosilylation,and then it was characterized by multiple surface analysis measurements including XPS,AFM,ellipsometry,and contact angle measurements etc.The results showed that there was a well-define and dense OEG monolayer on the silicon surface.Finally the experiments of fibrinogen adsorption with OEG monolayer were carried on,which showed that the densely OEG monolayer had a good protein-resistance ability,and moreover its degradation can be avoided under the physiological conditions after 28 days.According to these above results,we predict that it would be used widely in the research field of protein chip,bio-MEMS,biosensor,and drug delivery etc.