白纹伊蚊血淋巴游离氨基酸分析

Analysis of Free Amino Acids in Hemolymph of Aedes albopictus

用离心法收集羽化后24h内尚未取食的雌蚊血淋巴、吸正常鸡血届第4d和第11d的雌蚊血淋巴、吸受染疟原虫鸡血后第4d和第11d的雌蚊血淋巴,用日立835-50型氨基酸自动分析仪进行游离氨基酸的测定,并对结果做统计学分析。分析结果表明,所有雌蚊吸血后第4d血淋巴中18种游离氨基酸总量同羽化时相比下降,第11d有所回升,但仍低于羽化时的水平。吸正常鸡血后第4d,16种氨基酸含量降低,2种氨基酸含量增加;到第11d,同第4d相比,氨基酸含量有3种降低,15种增加。吸受染鸡疟原虫鸡血后第4d,14种氨基酸含量降低,4种氨基酸含量增加,到第11d,同第4d相比,氨基酸含量有7种降低,11种增加。

Hemolymph was collected from female Aedes albopictus by centrifugation. Collections were made from newly emerged mosquitoes, 4days and 11 days after mosquitoes had taken blood meal from Plasmodium gallinaceum-infected and noninfected chicks The result shows that the total amount of free amino acids (FAAs) in hemolymph of female Ae albopictus decreases 4days after blood meal and increases 11 days after the same meal, but is still less than that of newly emerged mosquitoes. The decrease of 16 FAAs and increase of 2FAAs were shown in the hemolymph of mosquitoes 4days after noninfected blood meal, the decrease of 3FAAs and increase of 15 FAAs were shown 11 days after the same meal. The decrease of 14 FAAs and increase of 4FAAs were shown in hemolymph 4days after infected blood meal, the decrease of 7 FAAs and increase of 11 FAAs were shown 11 days after the same meal.