生长激素释放多肽通过与生长激素释放因子不同的受体起作用

Effect and Mechanism of Growth Hormone-Releasing Factor to Growth Hormone Release

用固相法合成了生长激素释放因子(GRF),生长激素释放多肤(GHRP)和生长激素释放抑制因子(GRF-AN,D-Arg2-GRF44),并检测了多肽的生物学活性。结果表明1.GRF和GHRP在体内外均可刺激人垂体生长激素(GH)分泌。2.GRF和GHRP体外灌流时均可刺激牛垂体GH分泌。3.GHRP可促进大鼠生长发育。4.GRF-AN可抑制由GRF兴奋的人和动物垂体GH释放,但有较强的选择性,GRF-AN不抑制由GHRP兴奋的GH释放。表明,GRF和GHRP均可兴奋垂体GH分泌,但二者的机制有本质上的区别。

Growth hormone releasing factor(GRF), growth hormone releasing peptide (GHRP) and GRF-antagonist (GRF-AN, D-Arg2-GRF_(44)) were synthesized by solid phase method and their biological activities were detected in our institute. The result is reported as follows. 1.Both GHRP and GRF can stimulate human pituitory growth hormone (GH) secretion in vitro and in vivo.2.Both GHRP and GRF can stimulate bovine pituitory GH secretion in vitro,3.GHRP can accelerate immature rats growth 4 GRF-AN has a strong seletive inhibition on human and animal pituitory GH secretion stimulated by GRF and has no inhibition on GH secretion stimulated by GHRP. Our investigation indicates that both GRF and GHRP can stimulate pituitary GH secretion, but there is a essential distinction in mechanism between them.