癌转移抑制基因nm23在人肺癌中的转录表达研究

Transcriptional expression of metastasis suppression gene nm23 in human lung cancer

目的 探讨nm23基因在人肺癌发生、发展中的作用。方法 采用狭缝印迹杂交技术和非放射性地高辛标记检测系统,检测了143份不同部位、不同性质人肺组织中的nm23H1和nm23H2基因的mRNA表达。结果 nm23H1和nm23H2基因的mRNA表达均有从正常肺组织、肺良性病变组织、非癌肺组织、癌灶组织到癌转移淋巴结组织逐渐降低的趋势。其中,肺癌组织的nm23H2基因mRNA表达较正常肺组织显著较低(P<0.05),癌转移淋巴结组织的nm23H1和nm23H2基因mRNA表达均较正常肺组织显著降低(P<0.05)。肺癌组织的nm23基因表达与淋巴结有无癌转移无明显关系(P>0.05)。结论 nm23基因表达降低可能与肺癌发生有关。

Objective To investigate the role of nm23 gene in oncogenesis and progression of human lung cancer. Methods The mRNA expressions of nm23 H 1 and nm23 H 2 genes in a series of 143 pulmonary tissues collected at various sites and with different properties were studied with slot blot hybridization and non radioactive digoxigenin labeling and detection system. Results According to the observations on normal pulmonary tissues, benign lesions, para and non cancer tissues, primary cancer and metastatic lymph nodes, there was a tendency of mRNA expression reduction of nm23 H 1 and nm23 H 2 genes. Among them, nm23 H 2 mRNA expressions in lung cancer tissues were significantly decreased when compared with normal pulmonary tissues (P<0.05), and nm23 H 1 and nm23 H 2 gene expressions in metastatic lymph nodes were both reduced compared with those in normal pulmonary tissues (P<0.05). There was no significant correlation between nm23 gene expression and lymph node metastasis in lung cancer (P>0.05).Conclusion Reduction of nm23 gene expression might be associated with the oncogenesis of lung cancer, but no evidence of metastasis suppression by nm23 gene was revealed in this study.