冷冻肺癌细胞免疫增强作用的实验研究

A study on immunity enhancement of frozen human lung cancer cells in vitro 

目的 探讨冷冻免疫产生的机制。方法 通过人的肺癌细胞和自身淋巴细胞的体外混合培养,测定各组淋巴细胞转化率(LTR)及其培养上清液中可溶性白细胞介素2受体(SIL2R)和白细胞介素6(IL6)的表达,并用流式细胞仪(FCM)测定各组培养上清液对自身肺癌细胞增殖的影响。结果 ①冷冻组LTR及其培养上清液中SIL2R、IL6的含量明显高于对照组(P<0.01)。②冷冻组培养上清液对自身肺癌细胞的增殖有明显的抑制作用(P<0.01)。结论 ①冷冻肺癌细胞可促进淋巴细胞活化、增殖、分泌细胞因子。②冷冻激活免疫活性细胞,可明显抑制肺癌细胞的增殖。

Objective To investigate the mechanisms of cryo immunotherapeutic effect.Methods After the mixed culture of human lung cancer cells and autologous lymphocytes was conducted in vitro,lymphocyte transformation rate (LTR), soluble interleukin 2 receptor (SIL 2R) and interleukin 6 (IL 6) in the supernatants of the frozen lung cancer cells and the control were detected. Meanwhile, the effects of the supernatants of the culture of frozen lung cancer cells and the control on the proliferation of autologous lung cancer cells were also determined with FCM measurement.Results ①The levels of SIL 2R, IL 6 and LTR in the group of frozen lung cancer cells were all highly significant higher than those in the group of the control (P<0.01). ②The supernatant of the culture of the frozen lung cancer cells could remarkably inhibit the proliferation of the autologous lung cancer cells (P<0.01).Conclusion ①Frozen lung cancer cells may improve the immune function of lymphocytes through promoting their activation, proliferation and secretion of cytokines.②The activation of immune competent cells by freezing can remarkably inhibit the proliferation of lung cancer cells. Therefore, freezing can irritate the body's anti tumor immunity.