浒苔多糖对腹腔脓毒症大鼠的免疫调理作用

Immunomodulation effect of enteromorpha clathrata polysaccharides(ECP) on abdomen sepsis in rats

①目的研究浒苔多糖对腹腔脓毒症大鼠中性粒细胞和淋巴细胞及其亚群的影响,阐明浒苔多糖对腹腔脓毒症大鼠的免疫调理作用。②方法制作SD大鼠盲肠结扎穿孔(CLP)脓毒症模型,被分为假手术组、对照组和实验组,各20只。制作脓毒症模型后分别在3、12、24、48h采血待测。检查外周血涂片、白细胞总数、中性粒细胞、淋巴细胞计数和CD4+、CD8+T淋巴细胞亚群。③结果实验组白细胞计数和中性粒细胞计数比对照组明显降低,而淋巴细胞计数和CD4+T淋巴细胞水平比对照组明显升高(P<0.05)。④结论浒苔多糖可以明显降低脓毒症大鼠白细胞和中性粒细胞,从而减轻炎症反应;提高脓毒症大鼠淋巴细胞计数和CD4+T淋巴细胞,从而改善免疫功能。

Objective To knowledge immunomodulation effect of Enteromorpha clathrata polysaccharides （ECP） on neutrophil leukocyte and lymphocyte subsets in abdomen septic rats. Methods Sepsis model of cecal ligation - perforation （CLP） in SD rats were made and divided into three groups including sham oparation group （ n =20） , control group（ n =20） and experimental group（ n = 20）. Blood samples were collected ＇after CLP sepsis model at 3,12,24,48h respectively. Leukocyte, neutrophil leukocyte, lymphocyte counting and CD4 ＋ , CD8 ＋ T lymphocyte were detected. Results Leukocyte and neutrophil leukocyte in experimental group decreased more significantly than that in control group（ P 〈 0.05 ） , but lymphocyte and CD4 ＋ T lymphocyte in experimental group increased more significantly than that in control group（ P 〈 0.05）. Conclusions Enteromorpha clathrata polysaccharides can decrease leukocyte and neutrophil leukocyte in septic, rat and alleviate inflammatory reaction;while it can increase lymphocyte and CD4 ＋ Tlymphocyte and improve immune fimction.