一种解磷菌剂的研制及其应用效果

Development and application of a phosphate-dissolving bacteria agent

利用C53组合设计对5株具有较强溶磷能力的解磷细菌进行混合发酵,得到具有良好协同作用的菌株组合(Bc、Bs、Ap1),通过正交试验确定其降解土壤中难溶性磷的最佳接种量为Bc2%、Bs6%、Ap16%.按此接种比例,在解磷菌剂发酵培养基中接种各菌株,并将其在温度为30℃、摇床转速为140r?min-1条件下培养28h,即制得解磷菌剂,并进行白菜盆栽试验,结果表明该菌剂对白菜根长、株高及地上部鲜质量均具有良好的促进作用.

Five bacterial strains with strong phosphate-solubilizing ability were mixed fermentation by C53 composite design,and the co-fermentation systems consisted of the strains Bc,Bs,Ap1 were obtained.The optimum inoculation in degradation of insoluble phosphate in soil was developed through orthogonal design,and the best inoculation ratio was Bc 2%,Bs 6%,Ap1 6%.It was a phosphate-dissolving bacteria agent that the three strains were inoculated in the phosphate-dissolving bacteria fermentation medium according to above-mentioned inoculation ratio and cultured at 30 ℃ and shaking speed 140 r·min-1 for 28 h.Pot experiment results illustrated that the phosphate-solubilizing bacteria had a good advance on the plant height,root length and shoot fresh mass of Chinese cabbage.