偶氮氯膦Ⅰ分光光度法测定替米沙坦的含量

Spectrophotometric Method for the Determination of Telmisartan with ChlorophosphonazoⅠ

在pH=8.80的B-R弱碱性缓冲介质中,替米沙坦与偶氮氯膦Ⅰ染料反应形成离子缔合物,溶液颜色发生明显改变,最大吸收波长在558nm处,替米沙坦的浓度与溶液的变色程度呈良好的线性关系,从而建立了测定替米沙坦含量的光度法。在最大吸收波长处,替米沙坦的浓度在0～8.1×10-6mol/L范围内遵守比耳定律,表观摩尔吸光系数ε558为1.84×104L·mol-1·cm-1,检出限为8.63×10-7mol/L,研究了适宜的反应条件及影响因素。方法具有较高的灵敏度和良好的选择性,可用于市售药品、尿液及血浆中替米沙坦的测定。

In the Britton-Robinson buffer solution（pH=8.80）,telmisartan（TMST）could react with chlorophosphonazoⅠ（CPA Ⅰ）to form an ion-association complex.The colors of solutions changed obviously,and the maximum absorption wavelength was at 558nm.A sensitive spectrophotometric method was developed for the determination telmisartan.The Beer’s law was oheyed in the range of 0～ 8.1×10^-6 mol/L telmisartan.The apparent molar absorptivityε558was 1.84×10^4 L/（mol·cm）.The detection limit was 8.63×10^-7 mol/L.And optimum reaction conditions and influenced factors were investigated.The method had high sensitivity and fairly good selectivity,and has been applied to the determination of telmisartan in medicine samples,human serum and human urine.