胰液细胞学检查与PCR-SSP诊断胰腺癌之比较

Comparing cytologic examination of pure pancreatic juice with PCR-SSP for diagnosis of pancreatic carci-noma

目的评价胰液细胞学检测与聚合酶链反应一顺序特异性引物（ＰＣＲ－ＳＳＰ）检测Ｋ－ｒａｓ基因点突变诊断胰腺癌的价值。方法１．胰液离心沉淀物涂片，ＨＥ染色细胞学检查；２．采用针对胰腺癌Ｋ－ｒａｓ基因第１２位密码子点突变方式（ＯＧＴ、ＧＴＴ、ＧＡＴ）设计的顺序特异性引物（ＳＳＰ），对胰液标本进行聚合酶链反应（ＰＣＲ），产物借助常规电泳和染色检测有无Ｋ－ｒａｓ基因突变及突变方式。结果胰液细胞学检查对胰腺癌的确诊率为２３．６％（４／１７），ＰＣＲ－ＳＳＰ对胰腺癌的确诊率为９４．１％（１６／１７）。结论ＰＣＲ－ＳＳＰ检测胰液Ｋ－ｒａｓ基因突变诊断胰腺癌特异、敏感，其价值优于胰液细胞学检查。

Objective To evaluate the value of pancreatic juice in cytologic examination and PCR-SSSP indetecting K-ras gene mutation for diagnosis of pancreatic carcinoma. Methods (1) Cytologic examination of thePrecipitating of centrifugated pancreatic juice stained with hematoxylin and adn was done; (2) With regard to themutant styles CGT, and GAT at codon 12 of K-ras gene in pancreatic adenocarcinoma, the sequence-specialprimers (SSP) for polymerase chain reaction were used to study the mutations, and conventional gel electrophoresiswas performed for detection of mutations in the amplification products. Results The dianosis rate of pancreaticcarcinoma was 23.6% by cytologic examination, and 94.1% by PCR-SSP. Conclusion PCR-SSP for the diag nosis of pancreatic carcinoma is specific and sensitive, and much better than cyologic examination of pancreaticjuice.