兔胆色素结石中Ag、特异性IgG的检测及意义

Detection of antigen and antibody (spcific IgG) in experimental bile pigment stones formation in rabbits and their significance

目的探讨兔胆色素结石（ＰＳ）中是否含有抗原、特异性抗体及ＰＳ成因间的关系。方法选用家兔２０只，随机分成两组（１０×２），做胆石模型，胆道注人１２５Ｉ标记的灭活Ｅ．ｃｏｌｉ为实验组，而注人等量未标记的灭活Ｅ．ｃｏｌｉ为对照组，计算成石率，对实验组胆汁、胆石进行４０秒γ计数，胆石切片作抗３３６０Ｅ．ｃｏｌｉ特异ＩｇＧ荧光抗体染色，测定光强度。结果兔ＰＳ中有Ａｇ及特异性ＩｇＧ存在。结论ＰＳ中的Ａｇ及特异性ＩｇＧ可以形成免疫复合物（ＩＣ），而ＩＣ可能是ＰＳ的雏形，Ａｇ－Ａｂ亲和力可能是兔ＰＳ形成的力学机制之一。

Objective To investigate the relationship between antigen, antibody (specific IgG) and bilepigmental stone (PS) formation in rabbits. Method We calried out an animal model of PS formatiom in rab bits and divided them into two groups randomly with 10 each. We detected: (1)the rate of PS rormation; (2)theradioactivity of radiolabeled E. coli 3360 in PS and bile; (3) the quantity of specific IgG fluorescence on the slicesof PS. Result There were antigen and spcific IgG in the experimental PS. Conclusion The antigen and acting in the PS may form immune comune (IC) which may be the primitive form of experimental stone, and theaffinity of Ag-Ab may facilitate the formation of PS in rabbits.