兰州地区人群血清幽门螺杆菌致病因子抗体与上消化道疾病的关系

Study on the relationship between antibodies to Helicobacter pylori virulence factors and upper GI tract disease

目的研究兰州地区患者中幽门螺杆菌(Hp)致病因子VacA、CagA、UreA和UreB与上消化道疾病的关系。方法病理学HE染色诊断患者,免疫印迹技术检测80例慢性萎缩性胃炎(16例)、消化性溃疡(24例)及胃癌患者(40例)血清中VacA、CagA、UreA和UreB抗体,分析与疾病的相关性。结果兰州地区人群上消化道疾病患者中HpCagA、VacA、UreA和UreB抗体阳性率依次为87.50%、95.00%、82.50%和80.00%;消化性溃疡组UreB抗体阳性率显著低于胃癌组(P<0.05);在消化性溃疡、胃癌组中CagA抗体阳性率显著高于萎缩性胃炎组(P<0.05)。结论兰州地区上消化道疾病患者中Hp感染率高,以Ⅰ型菌株为主;UreB与胃癌的发病关系密切;CagA在消化性溃疡和胃癌发生中的作用更为明显。

Objective To study on the relationship between Helicobacter pylori（Hp） virulence factors,i.e.VacA,CagA,UreA,and UreB,and upper GI tract disease in the Lanzhou area.Methods HE staining was used to diagnose patients.Western blot was used to detect antibodies to VacA,CagA,UreA,and UreB in the sera of 80 patients with chronic atrophic gastritis（16 patients）,a peptic ulcer disease（24）,or gastric carcinoma（40） in order to analyze the relationship between virulence factors and the conditions in question.Results The sera of 80 patients with upper GI tract disease were positive for antibodies to CagA,VacA,UreA,and UreB at a rate of 87.50%,95.00%,82.50%,and 80.00%,respectively.The rate of sera positive for antibodies to UreB was significantly lower in the peptic ulcer group than in the gastric carcinoma group（P0.05）.The rate of sera positive for antibodies to CagA was significantly higher in the peptic ulcer group and gastric carcinoma group than in the chronic atrophic gastritis group（P0.05）.Conclusion There was a high rate of infection of Hp among people with an upper GI tract disease in the Lanzhou area,and most strains were type I.UreB may be closely related to the onset of gastric carcinoma.CagA clearly plays more of a role in the onset of peptic ulcers and gastric carcinoma.