荧光定量PCR检测精液HCMV感染及与不育相关性研究

Relationship between HCMV infections detected by fluorescence quantitative PCR and male infertility

目的采用荧光定量PCR检测精液内人巨细胞病毒(HCMV),探讨精液HCMV感染及与男性不育的相关性。方法采用荧光定量PCR技术检测85例男性不育患者精液HCMV感染情况,采用精子形态检测系统下人工修正方法对不育患者精液作常规检测及形态分析。结果 85例男性不育患者精液中HCMV阳性率为3.8%(3/85),与生育组(0/16)比较差异有统计学意义(P<0.05)。病毒感染组精子密度、精液pH值、精液量、液化时间、形态正常精子百分率与未感染组比较差异无统计学意义(P>0.05)。病毒感染组精子的头部畸形率显著高于未感染组(P<0.05)。结论 HCMV感染可能导致精子头部畸形,与男性不育的发生关系密切。

Objective To use fluorescence quantitative PCR to detect human cytomegalovirus（HCMV） in male infertility patients and examine the relationship between human cytomegalovirus infection and male infertility.Methods Fluorescence quantitative PCR detected 85 male infertility patients with HCMV infection.Sperm morphology and sperm vitality analysis were performed using a semiautomatic sperm morphology analyzer.Results Of 85 semen samples from patients,3.8%（3/85） were positive for HCMV,and this rate differed from that in virus-negative patients（0/16,P0.05）.Differences between the virus-positive patients and virus-negative patients in terms of the sperm concentration,semen pH,semen volume,liquefying time,and the percentage of sperm with normal morphology were not statistically significant（P0.05）.The percentage of sperm with an abnormal head was markedly higher in virus-positive patients than in virus-negative patients（P0.05）.Conclusion Infection with HCMV may lead to malformation of the head of sperm.Thus,HCMV infection may be closely correlated with male infertility.