盘基网柄菌发育期间蛞蝓体的形态学研究

Investigation on morphogenesis of slug during Dictyostelium discoideum development

用吖啶橙和Hoechst 33342两种活体荧光染料,通过荧光显微镜观察,来了解盘基网柄菌多细胞发育期间蛞蝓体阶段中出现的细胞分化及凋亡特点.结果发现:随着发育进程,将发育成柄细胞的前柄细胞先被染成蓝绿色,然后被染成绿色,再成橙色,最后为无色,这些分别表示了前柄细胞不同的凋亡阶段.在蛞蝓体后期其内部出现一条"通道".得出结论,蛞蝓体是盘基网柄菌细胞分化和衰退的起始阶段,其形态发生了巨大的变化.前柄细胞从蛞蝓体前期开始凋亡,但并不是马上死亡,而是一个渐进的凋亡过程.

The characters of cell differentiation and cell apoptosis of Dictyostelium discoideum, especially slug,were studied by Acridine orange and Hoechst 33342 staining,two vital fluorescence dyes,with the help of fluorescence microscopy.The results show that different degree apoptosis of the pre-stalk cells during development were referred to the colors,blue-green,green, orange,and finally colorless.In the later of slug stage,a channel-like structure were observed. These data indicate that slug is the initiation phase of cell differentiation and recessionary in Dictyostelium discoideum.Furthermore,the morphological characters change greatly during the slug stage.The pre-stalk cells have already initiated apoptosis since the early stage of slug period. However,their apoptosis is a gradual process.