HPLC法测定地锦草灌肠液中苦参碱的含量

Determination of grass enema Verbena matrine in by HPLC

目的:建立地锦草灌肠液中苦参碱的含量测定。方法:采用HPLC法,色谱柱为BDS Hypersil C18柱,流动相为甲醇-乙腈-三乙胺-磷酸盐缓冲液,流速为1.0 mL·min-1,检测波长为220 nm,柱温为室温,进样量为20μL。结果:苦参碱在0.3616-4.338μg范围内呈良好的线形关系(r=0.9996),平均回收率为97.7%,RSD%为0.77%(n=6)。结论:该方法简便,准确,重现性好,可用于地锦草灌肠液的质量控制。

Objective： To establish a creeper grass enema Determination of matrine.Methods： HPLC,column was BDS Hypersil C18 column with a mobile phase of methanol-acetonitrile-triethylamine-phosphate buffer,flow rate of 1.0 mL·min^-1,the detection wavelength was 220 nm,column temperature was room temperature,The injection volume was 20 μL.Results： matrine in the range 0.3616-4.338 μg showed a good linear relationship（r=0.9996）,the average recovery was 97.7%,RSD% was 0.77%（n=6）.Conclusion： The method is simple,accurate,reproducible,and can be used for Euphorbia humifusa enema quality control.