南瓜子叶再生体系的建立

Primary Establishment of Plant Regeneration System of Pumpkin

以5个南瓜品种为试材，比较了植物生长物质、基因型、子叶切割和接种方式等因素对不定芽分化的影响。结果表明：4～5d无菌苗的子叶沿中脉纵切，将其中脉斜插入培养基中，分化率最高。易于诱导不定芽的南瓜品种为“绿栗”南瓜和“夷香红栗”南瓜。“夷香红栗”南瓜的不定芽诱导最适培养基为MS＋6-BA3．0mg／L＋NAA0．1mg／L，不定芽分化率为37．5％，平均芽数2．2个；“绿栗”南瓜最佳培养方式为在MS＋6-BA3．0mg／L＋NAA0．1mg／L的培养基中培养12d，再转入MS＋6BA2．0mg／L＋GAa0．5mg／L上继续培养，不定芽分化率达到52％，平均芽数3．7个。

Taking five species pumpkin as test materials the effect of plant growth substances, genotype, cotyledon dissection and inoculation methods adventitious bud differentiation were studied. The results showed that the best regeneration rate has been achieved in cutting the cotyledon into half longitudinally of 4-5 day old seedlings, placing the middle leaf vein in the medium. The best genotypes were‘Luli ’ and ‘ Yixianghongli ’ to induce the adventitious bud among pumpkin species tested. The best medium composition for adventitious bud culture was MS＋6-BA 3. 0 mg/L＋NAA 0. 1 mg/L with adventitious bud differentiation ratio of 37. 5% ,producing average 2. 2 buds for ＇Yixianghongli＇. Whereas best medium composition for＇Luli＇was MS＋6-BA 3. 0 mg/L＋NAA 0. 1 mg/L for 12 days,and transferred into MS＋6-BA 2.0 mg/L＋NAA 0. 5 mg/L with the adventitious bud ratio of 52% ,average 3. 7 buds.